Dynamic temperature measurement in microfluidic devices using thermochromic liquid crystals |
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| Authors: | Hoang Viet N Kaigala Govind V Backhouse Christopher J |
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| Affiliation: | Applied Miniaturisation Laboratory, Department of Electrical and Computer Engineering, University of Edmonton, AB T6G 2V4, Canada. |
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| Abstract: | Thermochromic liquid crystals (TLCs) are used to explore the temperature transients during thermal cycling for microchip-based polymerase chain reaction (PCR). By analyzing the reflected spectra of the TLCs over time, temperature vs. time trajectories were extracted and overshoots/undershoots were estimated. To our knowledge, this is the first report of TLC-based dynamic temperature measurements in a microfluidic device for all PCR temperature stages. |
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