Label-acquired magnetorotation for biosensing: An asynchronous rotation assay |
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| Authors: | Ariel Hecht Paivo Kinnunen Brandon McNaughton Raoul Kopelman |
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| Institution: | 1. The University of Michigan, Department of Biomedical Engineering, 2200 Bonisteel, Ann Arbor, MI 48109-2099, United States;2. The University of Michigan, Department of Chemistry, 930 North University, Ann Arbor, MI 48109-1055, United States;3. The University of Michigan, Applied Physics Program, 2477 Randall Laboratory, Ann Arbor, MI 48109-1120, United States;1. School of Physics and Wuhan National High Magnetic Field Center, Huazhong University of Science and Technology, Wuhan 430074, People’s Republic of China;2. College of Electro-Information Engineering, Xuchang University, Xuchang 461000, People’s Republic of China;3. International Center of Materials Physics, Chinese Academy of Science, Shenyang 110015, People’s Republic of China;1. Department of Theoretical Physics, Faculty of Physics, Lomonosov Moscow State University, Moscow 119991, Russia;2. Joint Institute for Nuclear Research, Dubna 141980, Moscow Region, Russia |
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| Abstract: | This paper presents a novel application of magnetic particles for biosensing, called label-acquired magnetorotation (LAM). This method is based on a combination of the traditional sandwich assay format with the asynchronous magnetic bead rotation (AMBR) method. In label-acquired magnetorotation, an analyte facilitates the binding of a magnetic label bead to a nonmagnetic solid phase sphere, forming a sandwich complex. The sandwich complex is then placed in a rotating magnetic field, where the rotational frequency of the sandwich complex is a function of the amount of analyte attached to the surface of the sphere. Here, we use streptavidin-coated beads and biotin-coated particles as analyte mimics, to be replaced by proteins and other biological targets in future work. We show this sensing method to have a dynamic range of two orders of magnitude. |
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