The immobilized-enzyme stirrer: Part 2. Fluorimetric determination of ethanol with the use of immobilized alcohol dehydrogenase [1]

A novel fluorimetric method is described for the determination of ethanol by means of alcohol dehydrogenase immobilized on a stirrer. Ethanol is oxidized by alcohol dehydrogenase to aldehyde, with simultaneous conversion of NAD to NADH; the increase in fluorescence of NADH is measured at 465 nm (λ_ex = 370 nm). The calibration curve is linear for ethanol concentrations up to $\text{250 mg}\text{100 ml}$. Deproteinization is required prior to the assay of blood samples; recoveries are 97–102.7%. Enzyme stability, reproducibility and selectivity are discussed.