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共焦激光扫描荧光显微镜的扫描系统研究
引用本文:张平 陈德. 共焦激光扫描荧光显微镜的扫描系统研究[J]. 应用光学, 1995, 16(3): 33-38
作者姓名:张平 陈德
作者单位:华中理工大学光电子系
摘    要:本文简述共焦激光扫描灾光显微镜的基本原理,提出扫描系统的方案确定和优化设计的方法,使用该方法何使扫描渐晕减少为原来的1%。同时还给了扫描控制系统运行和控制法以及是均匀图的补偿方法。

关 键 词:共焦 激光显微镜 扫描 步进电机 显微镜

RESEARCH ON SCANNING SYSTEM OF CONFOCAL LASER SCANNING FLUORESCENCE MICROSCOPE
Zhang Ping, Chen De, Wu Zhen, Huang Jun. RESEARCH ON SCANNING SYSTEM OF CONFOCAL LASER SCANNING FLUORESCENCE MICROSCOPE[J]. Journal of Applied Optics, 1995, 16(3): 33-38
Authors:Zhang Ping   Chen De   Wu Zhen   Huang Jun
Affiliation:Huazhong University of Science and Technology
Abstract:The basical principle of confocal theer scanning fluorescence microscope is described briefly in the paper, and the method for determination of scanning system scheme and for optimum design robed. By use of this method, the scanning vignetting can be reduced to 1% of the original one. At the same time. the methods for operating and controlling the scanning controlled system and a compensation method for obtaining uniform images are also given.
Keywords:confocal laser microscope   seanning   fluorescece   vibrating mirror   step-motor
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