Nomographische und numerische Korrekturen für trübungsbedingte Untergrundabsorption bei spektralphotometrischen Gehaltsbestimmungen

It is shown by the example of cyanomethaemoglobin in agreement with the literature, that light absorption of protein solutions due to turbidity is—because of the size of the particles—only in the limiting case dependent on the Rayleigh Law. The validity to correct with an exponential formula for absorption due to turbidity is confirmed. Relations are derived, permitting to correct for background absorption by measuring the sample at three wavelengths without plotting: one nomographic method without neglections, an iterative numerical method and a linear approximation sufficiently accurate. These methods are universally applicable.