Measurement of Colloidal Stability in Solutions of Simple, Nonadsorbing Polyelectrolytes

The stability of a solution of charged polystyrene particles in the presence of nonadsorbing polyelectrolyte macromolecules is measured using optical light scattering. The particles were negatively charged polystyrene latex spheres (0.5–1 μm diameter) while the macromolecules were simulated using negatively charged colloidal silica spheres (5–7 nm diameter). Because of the electrostatic repulsion between the particles, the solution is found to be stable against primary flocculation (irreversible flocculation into a primary energy minima). However, because of long-range attractive depletion forces, reversible secondary flocculation of the particles occurs into a local potential energy minimum. As observed with uncharged macromolecules, the polyelectrolyte first induces flocculation at a critical flocculation concentration (v*), but later restabilizes the system at a critical restabilization concentration (v**). These critical concentrations are found to decrease with decreasing macromolecule size and increasing particle size. The restabilized solutions are found to remain suspended for periods greater than 20 days. Comparison of the measured flocculation and restabilization results to predictions made using a recently developed force-balance model show qualitative agreement.