甜茶苷的酶法制备及其对肝肠胃细胞的抑制作用

甜茶苷是一种自然界中含量稀少的甜味剂。 本实验从多种糖苷酶中筛选出一种来自Aspergillus niger的β-葡萄糖苷酶,用以特异性水解斯替夫苷而高通量制备甜茶苷。 最适条件下反应12 h后,甜茶苷的产率为90.4%,斯替夫苷的转化率达98.8%。 为了进一步考察甜茶苷的安全性并拓展其潜在的用途,实验考察了甜茶苷对人胃肠道和肝脏细胞的细胞毒性,即甜茶苷对3种正常细胞以及11种癌细胞生长的抑制效果。 实验结果表明,在250 μg/mL的质量浓度下,甜茶苷对正常人胃肠肝细胞无毒性;相同质量浓度下,甜茶苷对人体肝癌细胞BEL-7404的抑制率是5-氟尿嘧啶的30％。

Enzymatic Synthesis of Rubusoside and Its Inhibition on Liver and Gastrointestinal Cells

Rubusoside is a rare natural sweetener. In this experiment, a β-glucosidase from Aspergillus niger was screened from some glycosidases, which was used to proficiently produce rubusoside from stevioside. The optimum rubusoside yield and the stevioside conversion reach 90.4% and 98.8% in 12 h, respectively. Subsequently, the cytotoxicity of rubusoside on human gastrointestinal and liver cells was investigated, which includes 3 normal and 11 cancer cell lines. At a mass concentration of 250 μg/mL, rubusoside is not toxic to the normal cells, and its inhibition rate of rubusoside on BEL-7404 is 30% of that 5-fluorouracil proceeded.