|
|||||
|
|
Superactivity of peroxidase solubilized in reversed micellar systems |
|
| Authors: | L Setti P Fevereiro E P Melo P G Pifferi J M S Cabral M R Aires-Barros |
| Institution: | 1. Dipartimento di Chimica Industriale e dei Materiali, Bologna, Italy 2. Instituto de Tecnologia e Química Biológica, Universidade Nova Lisboa, Oeiras, Portugal 3. Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, 1000, Lisboa, Portugal |
| Abstract: | Vaccinium mirtyllus peroxidase solubilized in reversed micelles was used for the oxidation of guaiacol. Some relevant parameters for the enzymatic activity, such as pH,w o (molar ratio water/surfactant), surfactant type and concentration, and cosurfactant concentration, were investigated. The peroxidase showed higher activities in reversed micelles than in aqueous solution. The stability of the peroxidase in reversed micelles was also studied, namely, the effect ofw o and temperature on enzyme deactivation. The peroxidase displayed higher stabilities in CTAB/hexanol in isooctane reversed micelles, with halflife times higher than 500 h. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |