| 紫外-可见吸收光谱法研究细胞色素C活性中心铁离子与微量元素的相互作用 |
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| 引用本文： | 贾颖萍,郑学仿,胡皆汉. 紫外-可见吸收光谱法研究细胞色素C活性中心铁离子与微量元素的相互作用[J]. 光谱学与光谱分析, 2001, 21(2): 222-223 |
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| 作者姓名： | 贾颖萍 郑学仿 胡皆汉 |
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| 作者单位： | 1. 大连大学化学与化学工程系
2. 中国科学院大连化学物理研究所 |
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| 摘 要： | 应用紫外－可见吸收光谱法研究细胞细色素C（Fecty-C)活性中心铁离子在pH7．1的磷酸缓冲液中与4种微量元素M（Ⅱ）的直接相互作用。结果表明，细胞色素C（Fecyt-C)活性中心Fe(Ⅱ）离子可被微量元素M（Ⅱ）部分取代，形成部分的细胞色素C衍生物（Mcyt-C)，溶液的吸收光谱的Soret带最大吸收峰发生蓝移，且α带与β带最大吸收峰相对峰强减弱。
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| 关 键 词： | 细胞色素C 微量元素 紫外－可见吸收光谱 活性中心 金属酶 蓝移 相互作用 铁离子 |
| 修稿时间： | 2000-05-12 |
Study on Interaction between the Metal Ion in the Active Site of Cytochrome C and Trace Elements by Ultraviolet Spectroscopy |
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| Yingping JIA ,Xuefang ZHENG , and Jiehan HU. Study on Interaction between the Metal Ion in the Active Site of Cytochrome C and Trace Elements by Ultraviolet Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2001, 21(2): 222-223 |
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| Authors: | Yingping JIA Xuefang ZHENG Jiehan HU |
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| Affiliation: | Department of Chemistry and Chemical Engineering, Dalian University, 116622 Dalian. |
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| Abstract: | The direct interaction between the metal ion in the active center of cytochrome C(Fecyt-C) and four trace elements in pH 7.1 phosphate buffer by UV spectrometry has been studied. As a result, part of the metal ion Fe(II) in Fecyt-C was replaced and the corresponding cytochrome C derivatives were produced. The Soret absorption maximum is blue-shifted and the absorption coefficient of the alpha band relative to that of the beta band is decreased in comparison to the Fecyt-C. |
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| Keywords: | Cytochrome C Trace element Ultraviolet absorption spectroscopy |
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