核桃青皮不同极性酚类物质组成分析

作为核桃加工的副产物,核桃青皮所含不同极性酚类物质的组成不同,为了分级利用核桃青皮资源,采用正交试验优化核桃青皮酚类物质提取工艺,制备核桃青皮多酚粗提物,在此基础上,分别用水、石油醚、二氯甲烷以及乙酸乙酯分级萃取其中不同极性的酚类物质,收集乙酸乙酯萃取层和水层部分。采用高效液相色谱分析核桃青皮酚类物质粗提物的组成,用高分辨质谱对乙酸乙酯萃取层和水层部分的多酚组成进行分析。结果表明:乙酸乙酯萃取层样品中含有原儿茶醛、原儿茶酸、丁香酸、鞣花酸、儿茶素或表儿茶素、皮树脂醇、蓟黄素衍生物及三酰基葡萄糖；而水层样品中含有没食子酸酰葡萄糖、皮树脂醇以及短叶苏木酚羧酸。总的来说,核桃青皮含有的不同极性多酚类物质组成各异,可根据其组成特点进行分级利用,广泛用于各类食品或添加剂领域。

Composition Analysis of Phenolic Compounds with Different Polarities in Walnut Green Husk

As a by-product of walnut processing, walnut green husk contains different polar phenolic substances with different compositions. In order to grading utilization of walnut green husk resources, orthogonal test was used to optimize the extraction process of phenols and prepare phenols crude extracts from walnut green husk. On this basis, water, petroleum ether, dichloromethane and ethyl acetate were used to extract phenolic compounds with different polarities, and the ethyl acetate layer and water layer were collected separately. HPLC was used to analyze the composition of the crude extract and high resolution mass spectrometry was used to analyze the composition of the ethyl acetate layer and water layer. The results showed that the ethyl acetate layer sample contained protocatechuic aldehyde, protocatechuic acid, syringic acid, ellagic acid, catechin or epicatechin, skin resin alcohol, quercetin derivative and triacyl glucose. While the water layer samples contained picorealol, gallic acid gluconate, and short-leaved hematoxylin carboxylic acid. In general, phenolic compounds with different polarities in walnut green husk have different compositions, and could be used in grades according to its composition characteristics and widely used in various fields of food or additives.