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Fabrication of novel molecular recognition membranes by physical adsorption and self-assembly for surface plasmon resonance detection of TNT
Authors:Dhesingh Ravi Shankaran  Toshikazu Kawaguchi  Sook Jin Kim  Kiyoshi Matsumoto  Kiyoshi Toko  Norio Miura
Institution:1. Art, Science and Technology Center for Cooperative Research , Kasuga-shi, Fukuoka, 816-8580, Japan;2. Japan Society for the Promotion of Science , Tokyo, 102-8471, Japan;3. Art, Science and Technology Center for Cooperative Research , Kasuga-shi, Fukuoka, 816-8580, Japan;4. Graduate School of Agriculture, Kyushu University , Fukuoka, 812-8581, Japan;5. Graduate School of Information Science and Electrical Engineering , Kyushu University , Fukuoka, 812-8581, Japan
Abstract:Recent concern on international terrorism and weapons of mass destruction demands the development of novel analytical methods for identification and quantification of explosive molecules. In this article, we describe the development of high-performance immunosensors for detection of 2,4,6-trinitrotoluene (TNT), a prime component of the landmines and bombs used by terrorist and military forces. The immunosensors were constructed by physical adsorption and self-assembly methods, and their binding interactions with a monoclonal anti-TNT antibody were evaluated for TNT detection using the surface plasmon resonance technique. A home-made 2,4,6-trinitrophenyl-keyhole limpet hemocyanine conjugate was used for physical adsorption. A poly(ethylene glycol) hydrazine hydrochloride thiolate was used in the construction of self-assembled monolayer surface and was immobilized with trinitrophenyl-β-alanine by the amide coupling method. The immunosensors were highly selective, regenerable, rapid, and exhibited remarkable sensitivity down to the parts-per-trillion level for TNT by the indirect competitive inhibition principle.
Keywords:Immunosensor  Explosives  TNT  Surface plasmon resonance  Environmental monitoring
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