Comparison of two glutathione S -transferases used in capacitive biosensors for detection of heavy metals |
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| Authors: | Ebru SaatÇİ Mihaela Nistor Szilveszter Gáspár Elisabeth Csöregi Mesude İşcan |
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| Institution: | 1. Faculty of Arts and Sciences, Biology Department , University of Erciyes , 38039 Kayseri, Turkey saatci@erciyes.edu.tr;3. Department of Analytical Chemistry , University of Lund , PO Box 124, S-221 00, Getingev?gen 60, 222 41 Lund, Sweden;4. Faculty of Arts and Sciences, Biology Department , Middle East Technical University , 06031 Ankara, Turkey |
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| Abstract: | This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)6) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1?fM to 1?mM for Zn2+, and 10?pM to 1?mM for Cd2+. The GST-(His)6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1?fM to 10?µM, and Hg2+ in the range of 1?fM to 10?mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His)6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors for the detection of heavy metals. |
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| Keywords: | Capacitive biosensor 6His-tag glutathione S-transferase Bovine liver glutathione S-tranferase theta 2-2 Heavy-metal detection |
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