GasChromatography of Amino Acids as their N-Trifluoroacetyl Methyl Esters and Comparison with the Ion-exchange Method in Maize and Oats |
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Authors: | D. A. Shearer R. M. Warner |
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Affiliation: | Canada Department of Agriculture Chemistry , Biology Research Institute , Ottawa, Canada |
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Abstract: | A method for the preparation of N-trifluoroacetyl methyl esters of amino acids has been developed, permitting the separation and determination of 20 amino acids by temperature-programmed gas chromatography on two columns. The method was applied to the analysis of the amino acids in hydrolyzed maize and in hydrolyzed oats, where a direct comparison was made with the ion-exchange method. |
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Keywords: | conductometric sensor ammonium nonactin polyvinylchloride natural water analyses |
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