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GasChromatography of Amino Acids as their N-Trifluoroacetyl Methyl Esters and Comparison with the Ion-exchange Method in Maize and Oats
Authors:D A Shearer  R M Warner
Institution:Canada Department of Agriculture Chemistry , Biology Research Institute , Ottawa, Canada
Abstract:A method for the preparation of N-trifluoroacetyl methyl esters of amino acids has been developed, permitting the separation and determination of 20 amino acids by temperature-programmed gas chromatography on two columns. The method was applied to the analysis of the amino acids in hydrolyzed maize and in hydrolyzed oats, where a direct comparison was made with the ion-exchange method.
Keywords:conductometric sensor  ammonium  nonactin  polyvinylchloride  natural water analyses
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