Construction of an optical tweezer for nanometer scale rheology |
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Authors: | A Raghu Sharath Ananthamurthy |
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Institution: | 1. Department of Physics, Bangalore University, 560 056, Bangalore, India
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Abstract: | The optical tweezer is a versatile set-up that can be employed in a wide variety of studies investigating the microscopic
properties of materials. In particular, this set-up has in recent times been gainfully employed in probing rheological properties
of materials that exhibit viscoelasticity. These measurements can provide data at the micro and nanometer scales, not normally
accessible by rheometers that are used for measurements on bulk samples. In this work we describe a single laser beam optical
tweezer set-up, which is built around an inverted open microscope. The trapped polystyrene particle bead’s deviation from
the trap potential minimum is monitored by laser backscattering technique and the bead position measured by a quadrant photodiode
detector. Additionally, a provision is made for video microscopic studies on dispersed beads using a CCD camera. A single
particle microrheological experiment that can be performed using the set-up is described with relevant calculations. |
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