Force spectroscopy of single multidomain biopolymers: A master equation approach |
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Authors: | O?Braun Email author" target="_blank">U?SeifertEmail author |
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Institution: | 1.II. Institut für Theoretische Physik,Universit?t Stuttgart,Stuttgart,Germany |
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Abstract: | Experiments using atomic force microscopy for unfolding single multidomain biopolymers cover a broad range of time scales
from equilibrium to non-equilibrium. A master equation approach allows to identify and treat coherently three dynamical regimes
for increasing linear ramp velocity: i) an equilibrium regime, ii) a transient regime where refolding events still occur,
and iii) a saw-tooth regime without any refolding events. For each regime, analytical approximations are derived and compared
to numerically investigated examples. We analyze in the framework of this model also a periodic experimental protocol instead
of a linear ramp. In this case, a major simplification arises if the dynamics can be restricted to an effectively two-dimensional
subspace. For transitions with an intermediate meta-stable state, like Immunoglobulin27, a refined model allows to extract
previously unknown molecular parameters related to this meta-stable state. |
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Keywords: | 87 15 -v Biomolecules: structure and physical properties 87 64 Dz Scanning tunneling and atomic force microscopy |
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