Spectrophotometric determination of uric acid based on fading of o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethyl-ammonium complex. |
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| Authors: | Takako Yamaguchi Kohei Hasegawa Shinichiro Kamino Kanako Miyachi Hiroshi Tominaga Yoshikazu Fujita |
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| Affiliation: | Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan. |
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| Abstract: | A simple and highly sensitive spectrophotometric method for the determination of uric acid (UA) was established based on fading of the o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex. In the determination of UA, Beer's law is obeyed in the range of 0.01-0.20 microg ml(-1), with an effective molar absorptivity at 635 nm, the relative standard deviation being 6.5 x 10(5) dm(3) mol(-1) cm(-1) and 1.5% (n = 5). This method is about 20-times more sensitive than the conventional methods. The method was successfully applied to the assay of UA in human urine. |
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