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STUDY ON PREPARATION AND APPLICATION OF MONOCLONAL ANTIBODIES TO Anabaena azollae
作者姓名:刘中柱  程由铨  唐龙飞  郑琦  宋铁英  陈明昂  李怡英  林天龙
作者单位:Fujian Academy of Agricultural Sciences,Fuzhou,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian Academy of Agricultural Sciences,Fuzhou
摘    要:Forty hybridoma cell lines secreting monoclonal antibodies against Anabaena azollae have been established by fusing SP_2/O myeloma cells with spleen cells from BALB/c mice immunized with purified antigen Anabaena azollae from different strains of azolla.According to the fluorescent antibody test, 40 McAbs were divided into 8 types. They may differentiate the Anabaena azollae in 195 azolla strains which are collected from different places in the world, into 8 antigenic groups.The identifying result of Anabaena azollae isolated from reconstituted azolla and sexual hybrid azolla showed that the Anabaena azollae in the reconstituted azolla retains its antigenicity from the donor azolla, and Anabacna azollae in hybrid azolla maintains its antigenicity from the female parent plant.


STUDY ON PREPARATION AND APPLICATION OF MONOCLONAL ANTIBODIES TO Anabaena azollae
LIU ZHONG-ZHU.STUDY ON PREPARATION AND APPLICATION OF MONOCLONAL ANTIBODIES TO Anabaena azollae[J].Science in China(Chemistry),1989(5).
Authors:LIU ZHONG-ZHU
Abstract:Forty hybridoma cell lines secreting monoclonal antibodies against Anabaena azollae have been established by fusing SP_2/O myeloma cells with spleen cells from BALB/c mice immunized with purified antigen Anabaena azollae from different strains of azolla.According to the fluorescent antibody test, 40 McAbs were divided into 8 types. They may differentiate the Anabaena azollae in 195 azolla strains which are collected from different places in the world, into 8 antigenic groups.The identifying result of Anabaena azollae isolated from reconstituted azolla and sexual hybrid azolla showed that the Anabaena azollae in the reconstituted azolla retains its antigenicity from the donor azolla, and Anabacna azollae in hybrid azolla maintains its antigenicity from the female parent plant.
Keywords:Anabaena azollae  monoclonal antibody    
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