Determination of tolperisone enantiomers in plasma and their disposition in rats. |
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| Authors: | T Yokoyama K Fukuda S Mori M Ogawa K Nagasawa |
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| Institution: | Department of Hospital Pharmacy, Kyoto Pharmaceutical University, Japan. |
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| Abstract: | A stereoselective assay for the determination of tolperisone enantiomers in plasma by high performance liquid chromatography was developed. Calibration curves obtained for the enantiomers were linear over plasma concentrations of 0.1-3.0 micrograms/ml with a detection limit of 20 ng/ml. Following intravenous bolus administration of 10 mg/kg of racemic tolperisone to rats, stereoselective disposition of tolperisone enantiomers was observed, and plasma concentrations were significantly higher for l-tolperisone than for d-tolperisone at 5, 15 and 30 min after administration. When either enantiomer was administered alone to rats, both enantiomers were found in plasma, indicating that a mutual chiral inversion occurs in the body. |
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