西洋参和高丽白参的X射线显微鉴定研究

传统的中药材显微鉴定,样品制备苛刻.加入某些试剂容易造成结构信息丢失.本文利用X射线相位衬度成像方法较为系统地研究了西洋参、高丽白参的显微结构,并对二者在草酸钙簇晶、导管、木栓细胞、韧皮部和木质部射线等显微结构方面的异同进行了详细分析.实验研究结果表明,该方法能够在无需对样品进行特殊处理的情况下,较好地实现西洋参、高丽白参的显微结构鉴定,有望成为中药材显微鉴定领域中一种简便快速的新型鉴定方法.实验还发现,西洋参和高丽白参韧皮部和木质部射线的显微结构存在很大的区别.这一显微结构有可能成为人参类贵重中药材显微鉴别中的一个新增依据.

Microscopic Identification of Panax Quinquefolium and Panax Ginseng by X-Ray Phase Contrast Imaging

In the conventional microscopic identification of traditional Chinese medicines (TCMs), disadvantages, such as rigid sample preparations and adding extra reagents which may result in structure information loss limit its application. X-ray phase contrast imaging (XPCI) was introduced to study the microscopic identification of Panax quinquefolium and Panax ginseng, in the aspects of clusters of calcium oxalate, vessels, cork cells, phloem and xylem rays. The experimental results show that XPCI method is convenient for microscopic identification. It is expected that XPCI will provide a new practical method for the identification of TCMs without any special sample treatments. The experiments also reveal that the phloem and xylem rays of Panax quinquefolium and Panax ginseng are obviously different. This microstructure difference may add another important basis for the microscopic identification of different ginsengs.