响应曲面法用于高效液相色谱优化分离磺胺的研究

通过响应曲面法对高效液相色谱分离磺胺的优化过程进行了研究,分别考察了分离的3个主要因素(柱温、流动相配比、流速)对最小分离度与分离时间的影响,建立了3个因素与最小分离度和最大保留时间的目标函数,从响应曲面图可优化预测因素水平范围内的最佳分离条件.在选定优化条件下:柱温为20℃,乙腈的体积分数19%,流速1.5 mL/min,8种磺胺组分可在8 min内达到基线分离.

Application of response surface methodology in optimizing separation of sulfonamides by high performance liquid chromatography

The separation of eight sulfonamides was studied using response surface methodology by high performance liquid chromatography.The effect of three factors on the minimum resolution and the retention time of the last eluted component was investigated such as column temperature,mobile phase percentage and flow rate.The objective equations have been established and the response surface curves and their respective contours have been plotted,so that the optimum conditions could be obtained.Baseline-separation of eight sulfonamides could be obtained at a 19% ACN aqueous solution(v/v),the flow rate of 1.5 mL/min during 8 min.