| 德国小蠊变应原Bla g 2蛋白变复性的荧光光谱研究 |
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| 引用本文： | 朱健琦,刘志刚,黄海珍,朱永烽,徐宏.德国小蠊变应原Bla g 2蛋白变复性的荧光光谱研究[J].光谱学与光谱分析,2006,26(12):2254-2257. |
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| 作者姓名： | 朱健琦 刘志刚 黄海珍 朱永烽 徐宏 |
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| 作者单位： | 深圳大学生命科学学院,广东,深圳,518060;中国科学院应用化学研究所电分析化学国家重点实验室,吉林,长春,130022 |
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| 基金项目： | 国家自然科学基金
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广东省科技厅科技计划
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广东省深圳市科技计划 |
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| 摘 要： | 包涵体中的重组蛋白经抽提后可以在变性状态下纯化，而纯化后的复性过程是基因工程下游处理的重要环节。通过对变应原Bla g 2蛋白复性前后荧光光谱的比较和分析、变性剂(尿素和SDS)对复性后Bla g 2蛋白的荧光滴定实验、以及复性后Bla g 2在不同pH下的荧光光谱分析，推断出Bla g 2蛋白分子在不同环境下构象的变化及其光谱学特征，初步建立了一种新的检测重组变应原蛋白变复性的光谱实验方法。
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| 关 键 词： | 德国小蠊 变应原 Bla g 2 变性 复性 荧光光谱 |
| 文章编号： | 1000-0593（2006）12-2254-04 |
| 收稿时间： | 2005-11-06 |
| 修稿时间： | 2006-02-20 |
Study of Fluorescence Spectra of Blattela Germanica Allergen,Derenatured and Renatured Bla g 2 |
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| ZHU Jian-qi , LIU Zhi-gang , HUANG Hai-zhen, ZHU Yong-feng , XU Hong.Study of Fluorescence Spectra of Blattela Germanica Allergen,Derenatured and Renatured Bla g 2[J].Spectroscopy and Spectral Analysis,2006,26(12):2254-2257. |
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| Authors: | ZHU Jian-qi LIU Zhi-gang HUANG Hai-zhen ZHU Yong-feng XU Hong |
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| Institution: | 1. College of Life Science, Shenzhen University, Shenzhen 518060, China2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China |
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| Abstract: | Recombinant proteins extracted from inclusion body remain in denaturation status. Renaturation in vitro after initial purification is a key step of downstream processing. A common method of renaturation of recombinant proteins is the dilution method. With Bla g 2 as a model protein, the conformational changes of denatured and renatured Bla g 2 were investigated by applying fluorescence spectra. The effects of different urea concentrations, different SDS concentrations and different pH on the fluorescence intensity of renatured protein were also investigated. The reasons for these were studied with the knowledge of molecular structure. |
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| Keywords: | Blattella germanica Allergen Bla g 2 Denaturation Renaturation Fluorescence spectra |
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