Preparation and a time-resolved fluoroimmunoassay application of new europium fluorescent nanoparticles. |
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Authors: | Xiaodan Hai Mingqian Tan Guilan Wang Zhiqiang Ye Jingli Yuan Kazuko Matsumoto |
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Affiliation: | Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, PR China. |
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Abstract: | New silica-based europium fluorescent nanoparticles having surface amino groups were prepared by a covalent binding-copolymerization technique. In the nanoparticles, the fluorescent Eu3+ chelate molecules were covalently bound to silicon atoms to protect the nanoparticles from dye leaking in bio-applications. The amino groups on the surface of nanoparticles made the surface modification and bioconjugation of nanoparticles easier. The nanoparticles were characterized and developed as a new type of fluorescence probe for a highly sensitive time-resolved fluoroimmunoassay (TR-FIA) of human hepatitis B surface antigen (HBsAg). |
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