| Study on an electrochemical biosensor for thrombin recognition based on aptamers and nano particles |
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| 作者单位： | ZHENG Jing（Department of Chemistry, East China Normal University, Shanghai 200062, China） ;LIN Li（Department of Chemistry, East China Normal University, Shanghai 200062, China） ;CHENG GuiFang（Department of Chemistry, East China Normal University, Shanghai 200062, China） ;WANG AnBao（Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China） ;TAN XueLian （Department of Chemistry, East China Normal University, Shanghai 200062, China） ;HE PinGang（Department of Chemistry, East China Normal University, Shanghai 200062, China） ;FANG YuZhi（Department of Chemistry, East China Normal University, Shanghai 200062, China） ; |
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| 摘 要： | This paper presents a high specific, sensitive electrochemical biosensor for recognition of protein such as thrombin based on aptamers and nano particles. Two different aptamers were chosen to construct a sandwich manner for detecting thrombin. Aptamer I was immobilized on nano magnetic particle for capturing thrombin, and aptamer II labled with nano gold was used for detection. The electrical current generated from gold after the formation of the complex of magnetic particle, thrombin and nano gold, and then an electrochemical cell designed by ourselves was used for separating, gathering, and electrochemical detecting. Through magnetic separation, high specific and sensitive detection of the target protein, thrombin, was achieved. Linear response was observed over the range 5.6×10-12―1.12×10-9 mol/L, with a detection limit of 1.42×10-12 mol/L. The presence of other protein as BSA did not affect the detection, which indicates that high selective recognition of thrombin can be achieved in complex biological samples such as human plasma.
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| 收稿时间： | 2005-12-07 |
| 修稿时间： | 2006-08-23 |
Study on an electrochemical biosensor for thrombin recognition based on aptamers and nano particles |
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| Zheng Jing,Lin Li,Cheng GuiFang,Wang AnBao,Tan XueLian,He PinGang,Fang YuZhi. Study on an electrochemical biosensor for thrombin recognition based on aptamers and nano particles[J]. Science in China(Chemistry), 2007, 50(3): 351-357. DOI: 10.1007/s11426-007-0062-4 |
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| Authors: | Zheng Jing Lin Li Cheng GuiFang Wang AnBao Tan XueLian He PinGang Fang YuZhi |
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| Affiliation: | 1. Department of Chemistry, East China Normal University, Shanghai 200062, China
2. Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China |
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| Abstract: | This paper presents a high specific, sensitive electrochemical biosensor for recognition of protein such as thrombin based on aptamers and nano particles. Two different aptamers were chosen to construct a sandwich manner for detecting thrombin. Aptamer I was immobilized on nano magnetic particle for capturing thrombin, and aptamer II labled with nano gold was used for detection. The electrical current generated from gold after the formation of the complex of magnetic particle, thrombin and nano gold, and then an electrochemical cell designed by ourselves was used for separating, gathering, and electrochemical detecting. Through magnetic separation, high specific and sensitive detection of the target protein, thrombin, was achieved. Linear response was observed over the range 5.6×10−12−1.12×10−9 mol/L, with a detection limit of 1.42×10−12 mol/L. The presence of other protein as BSA did not affect the detection, which indicates that high selective recognition of thrombin can be achieved in complex biological samples such as human plasma. |
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| Keywords: | aptamers nano particles protein electrochemical sensor |
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