| Abstract: | Dark-grown cells of Euglena gracilis var. bacillaris incubated on resting medium for 7 days lose half of their extractable and phototransformable protochlorophyll(ide) [Pchl(ide)]. The readily-utilized substrates glutamate and malate bring about a regeneration of both pools of Pchl(ide) to the levels found in growing cells, without causing cell division during the period of regeneration. Light does not cause a regeneration of total extractable Pchl(ide) to the levels found in growing cells, and the complete regeneration of phototransformable Pchl(ide) seen probably results from conversion of non-transformable Pchl(ide) to transformable Pchl(ide). Optimal glutamate, malate, and light in any combination are no better in causing phototransformable Pchl(ide) regeneration than any one treatment alone, indicating that the Pchl(ide) pool size is limited by other factors. The regeneration of phototransformable Pchl(ide) induced by light or substrates is insensitive to inhibitors of protein synthesis on 80 S cytoplasmic ribosomes (cycloheximide) or on 70 S chloroplast ribosomes (streptomycin). Cycloheximide, however, induces the regeneration of phototransformable Pchl(ide) in darkness in the absencc of externally-added substrates. Since cycloheximide is known to induce paramylum breakdown under the same conditions, it is likely that this process can provide internal substrates and/or reducing power for phototransformable Pchl(ide) regeneration. Possible mechanisms of regulation of formation of Pchl(ide)635 in Euglena are discussed and compared with regulation of Pchl(ide)650 in higher plants. |
