| A Novel β-1, 4-N, 6-O-Diacetylmuramidase from Streptomyces griseus and its Chemical Modification |
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| 作者姓名： | Chang Kui SUN Yu Zhen ZHANG Ming CHEN |
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| 作者单位： | Chang Kui SUN，Yu Zhen ZHANG，Ming CHEN State Key Laboratory of Microbial Technology，Shandong University，Ji'nan 250100 |
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| 基金项目： | Acknowledgments The authors were grateful to the National Natural Science Foundation of China （No. 30470050） and Natural Science Foundation of Shandong Province （No. z2005d02） for financial support, |
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| 摘 要： | A novel lysozyme namedβ-1, 4-N, 6-O-diacetylmuramidase R2 was purified and characterized from Streptomyces griseus. The molecular weight of the enzyme was determined by MALDI-TOF-MS as 23.5 kDa. The N-terminal amino acid sequence was DTSGVQGIDVS-HWQG.Chemical modification ofβ-1, 4-N, 6-O-diacetylmuramidase R2 indicated that sulfhydry1 group and carbamidine of arginine residues are not essential for the activity of the enzyme, but lysine residues and imidazole of histidine residues are essential for the activity. The number of essential tryptophan and carboxyl groups was found that only one tryptophan residue and three carboxyl groups in the active site.
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| 关 键 词： | 化学修饰 溶菌酶 细胞序列 化学分析 |
| 收稿时间： | 2006-03-23 |
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