Bioconversion of fumarate to succinate using glycerol as a carbon source |
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Authors: | Hwa-Won Ryu Kui-Hyun Kang Jong-Sun Yun |
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Institution: | (1) Department of Biochemical Engineering, Chonnam National University, 500-757 Kwangju, Korea;(2) Department of Chemical Engineering, Chonnam National University, 500-757 Kwangju, Korea;(3) Department of Chemical Technology, Chonnam National University, 500-757 Kwangju, Korea |
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Abstract: | In this study, a facultative bacterium that converts fumarate to succinate at a high yield was isolated. The yield of biocon
version was enhanced about 1.2 times by addition of glucose into culture medium at an initial concentration of 6 g/L. When
the initial cell density was high (2 g/L), the succinate produced at pH 7.0 for initial fumarate concentrations of 30, 50,
80, and 100 g/L were 29.3, 40.9, 63.6, and 82.5 g/L, respectively, showing an increase with the initial fumarate concentration.
The high yield of 96.8%/mole of fumarate in just 4 h was obtained at the initial fumarate concentration of 30 g/L. Comparing
these values to those obtained with low cell culture (0.2 g/L), we found that the amount of succinate produced was similar,
but the production rate in the high cell culture was about three times higher than was the case in the low cell culture. This
strain converted fumarate to succinate at a rate of 3.5 g/L·h under the sparge of CO2. |
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Keywords: | Bioconversion succinate fumarate Enterococcus sp RKY1 fumarate reductase |
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