Label-free fluorescence detection in capillary and microchip electrophoresis |
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Authors: | Philipp Schulze Detlev Belder |
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Institution: | (1) Institute of Analytical Chemistry, Universitat Leipzig, Johannisallee 29, 04103 Leipzig, Germany |
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Abstract: | Herein, we summarize the current status of native fluorescence detection in microchannel electrophoresis, with a strong focus
on chip-based systems. Fluorescence detection is a powerful technique with unsurpassed sensitivity down to the single-molecule
level. Accordingly fluorescence detection is attractive in combination with miniaturised separation techniques. A drawback
is, however, the need to derivatize most analytes prior to analysis. This can often be circumvented by utilising excitation
light in the UV spectral range in order to excite intrinsic fluorescence. As sensitive absorbance detection is challenging
in chip-based systems, deep-UV fluorescence detection is currently one of the most general optical detection techniques in
microchip electrophoresis, which is especially attractive for the detection of unlabelled proteins. This review gives an overview
of research on native fluorescence detection in capillary (CE) and microchip electrophoresis (MCE) between 1998 and 2008.
It discusses material aspects of native fluorescence detection and the instrumentation used, with particular focus on the
detector design. Newer developments, featured techniques, and their prospects in the future are also included. In the last
section, applications in bioanalysis, drug determination, and environmental analysis are reviewed with regard to limits of
detection. |
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Keywords: | Native (intrinsic) fluorescence Optical detection Microfluidic chip Capillary electrophoresis Miniaturisation |
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