| Cloning, Sequencing and Expression in E. coli of Interferon-ω1 Gene |
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| 作者姓名： | 黎孟枫 曾庆 周园 郭辉玉 侯云德 |
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| 作者单位： | Institute of Virology,Chinese Academy of Preventive Medicine,National Laboratory of Molecular Virology,Beijing 100052,PRC,Institute of Virology,Chinese Academy of Preventive Medicine,National Laboratory of Molecular Virology,Beijing 100052,PRC Guangdong Inatiture of Microbiology,Guangzhou 510070,PRC,Institute of Virology,Chinese Academy of Preventive Medicine,National Laboratory of Molecular Virology,Beijing 100052,PRC,Institute of Virology,Chinese Academy of Preventive Medicine,National Laboratory of Molecular Virology,Beijing 100052,PRC Department of Microbiology & Innunology,Sun Yat-sen University of Medical Sciences,Guangzhou 510089,PRC,Institute of Virology,Chinese Academy of Preventive Medicine,National Laboratory of Molecular Virology,Beijing 100052,PRC |
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| 基金项目： | This work was supported by the National "863" Project for Biotechnology Development. |
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| 摘 要： | Human interferon ω1 (huIFN-ω1) gene was isolated and cloned from chromosome DNA derived from a Chinese fetal liver via polymerase chain reaction (PCR). By determining its nucleotide sequence we proved that the 88th codon should be GGA, coding for Gly. After engineering the original IFN-ω1 gene clone to a form that may be expressed as a nonfused protein, we also took the IFN-ω1 gene under the control of the PRPL promoter with an expression vector pBV220 in E. coli. The antivirus activity of the recombinant IFN-ω1 is about 6.5×10~7 units/L CULTURE (OD_(600)=0.75). Since IFN-ω1 not only has antivirus activity but also shows considerably high homology with animal trophoblast proteins which have been proved antiluteolysins as a maternal recognition signal for pregnancy, we believe that study on it will be practically and theoretically significant.
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