Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection |
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| Authors: | Yang C Mertz J |
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| Affiliation: | Laboratoire de Neurophysiologie et Nouvelles Microscopies, Ecole Supérieure de Physique et Chimie Industrielles, Institut National de la Santé et de la Recherche Médicale EPI-0002, Paris, France. |
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| Abstract: | We present a transmission-mode confocal laser scanning microscope system based on the use of second-harmonic generation (SHG) for signal detection. Our method exploits the quadratic intensity dependence of SHG to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal acts as a virtual pinhole that remains self-aligned without the need for descanning. |
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