| Abstract: | This paper describes a new method for gradient elution at low flow rate. By adjusting the solvent composition flowing into a dynamic mixer, we can program the concentration of solvent, B, inside a dynamic mixer to form a time-resolved solvent gradient delivered to the column. The input gradient is related to the output gradient by the equation, Bin = Bout + τ × dBout/dt, in which τ is the gradient response time constant at specified flow rate, and Bin and Bout are input and output concentration of solvent B, respectively. |
