| Abstract: | A novel technique of preparation of multilayer assemblies consisting of a controlled number of molecular layers of various proteins arranged according to a predetermined architecture is described. The multilayers were formed by consecutive adsorption of polyanions and positively charged proteins below their isoelectric point and covalently crosslinked via protein amino groups. Polyanions were washed out from the crosslinked protein assembly by rinsing with buffer in which the net charge of the proteins was inverted. |
