| Abstract: | To develop photo-tunable chromatography, several azobenzene derivatives were immobilized or modified on silica gels or incorporated into polymers. The supports prepared were used as stationary phases in semi-micro HPLC, and the photo-dependence of their retention evaluated. On these stationary phases, the retention measured with 2-propanol in hexane was increased by UV irradiation; in contrast, the retention was decreased when methanol was used as mobile phase. It was assumed that the polarity of the stationary phase increases when the molecular structure of the azobenzene moieties changes from the trans to the cis form. On irradiation of the column with visible light after UV irradiation and with methanol as mobile phase, the change of retention proved to be reversible. However, this reverse change was less dramatic than the initial change. Such retention behavior is attributable to photo-isomerization of the azobenzene moieties. These findings will be exploited in our development of new photo-tunable stationary phases and new photo-controlled separation systems. |
