The Utility of Capped Duplex DNA (σ-DNA) in Triplex Interaction Studies

Two optimally capped duplex DNA molecules (σ-DNA; see 1 and 4 ) were synthesized and their utility demonstrated for triplex investigations with their corresponding homopyrimidine DNA and RNA single strands in the D⋅(D⋅D) and R⋅(D⋅D) Hoogsteen mode. Furthermore, it was established that σ-DNA is an ideal tool to study the pH dependency of triplex formation.