New separation method for organic and inorganic selenium compounds based on anion exchange chromatography followed by inductively coupled plasma mass spectrometry |
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Authors: | L Ayouni F Barbier J L Imbert J Y Gauvrit P Lantéri M F Grenier-Loustalot |
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Institution: | (1) CNRS USR 59, Service Central d’Analyse, Chemin du Canal, BP 22, 69390 Vernaison, France;(2) UMR 5180, Laboratoire de Chimiométrie, Université Claude Bernard Lyon1, 43, Bd du 11 novembre 1918, Batiment 308D, 69622 Villeurbanne, France |
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Abstract: | We describe a new method for separating the organic and inorganic selenocompounds methaneseleninic acid, selenite, selenate,
methylselenocysteine, selenocystine as well as both selenomethionine and its oxidized form. The separation is performed on
a Hamilton PRP-X100 column. According to the literature, the oxidized form of selenomethionine—which is easily formed—is eluted
close to the dead volume when this column is used. The choice of parahydroxybenzoic acid as mobile phase enabled us to elute
all of these species after this oxidized form, resulting in better identification and quantification. The factors determining
separation (eluent concentration, pH, gradient) were optimized via an experimental design. Application of the method to yeast
and commercial tablets showed that the principal Se compound present was selenomethionine, which was also present in its oxidized
form. |
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Keywords: | Selenium Speciation Anion exchange chromatography Experimental design |
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