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Blotting Assisted by Heating and Solvent Extraction for DESI-MS Imaging |
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| Authors: | Elaine C. Cabral Mario F. Mirabelli Consuelo J. Perez Demian R. Ifa |
| Affiliation: | 1. Centre for Research in Mass Spectrometry, York University, Toronto, Ontario, Canada 2. University of Calabria, Via P. Bucci, Arcavacata di Rende, Cosenza, Italy |
| Abstract: | Imprints of potato sprout (Solanum tuberosum L.), gingko leaves (Gingko biloba L.) and strawberries (Fragaria x ananassa Duch.) were successfully imaged by desorption electrospray ionization mass spectrometry (DESI-MS) on TLC plates through blotting assisted by heating and/or solvent extraction. Ion images showing the distribution of significant compounds such as glycoalkaloid toxins in potato sprout, ginkgolic acids and flavonoids in ginkgo leaves, and sugars and anthocyanidin in strawberry were obtained. Practical implications of this work include analysis of a wide range of irregular or soft materials by different imprinting conditions without requiring the addition of matrices or use of specific kinds of surfaces. DESI-MS Imaging analysis of gingko leaves imprinted onto TLC plates. The ion image shows the spatial distribution of quinic acid ion (m/z 191). |
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