Accelerated extraction for determination of polycyclic aromatic hydrocarbons in marine biota |
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Authors: | J Sanz-Landaluze L Bartolome O Zuloaga L González C Dietz C Cámara |
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Institution: | (1) Department of Analytical Chemistry, Faculty of Chemistry, University Complutense de Madrid, Ciudad Universitaria, 28040 Madrid, Spain;(2) Department of Analytical Chemistry, Faculty of Technology, Euskal Herriko Unibertsitatea, 644 P.K., 48080 Bilbao, Spain |
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Abstract: | A rapid and simple method is proposed for determination of polycyclic aromatic hydrocarbons (PAH) in complex matrices such
as marine biota. The method uses sonication, by means of an ultrasonic probe, as a new tool for assisted extraction, coupled
with reversed-phase liquid chromatography (RP-LC) with fluorescence detection (FL) for determination of 16 US EPA priority
PAH. Separation and detection of the 16 PAH were complete in 45 min by RP-LC with a C18 column and acetonitrile–water gradient elution. Multivariate optimisation of the variables affecting extraction (ultrasound
radiation amplitude, sonication time, and temperature of the water-bath in which the extraction cell was placed) was conducted.
The accuracy of the method was determined by analysis of a certified reference material and comparison of the results obtained
with those from another method (microwave-assisted extraction and GC–MS). The new technique avoids the main problems encountered
in the determination of PAH in complex matrices such as marine biota, and no clean-up step is necessary. The method was applied
to determination of PAH in estuarine biota samples from the Urdaibai estuary (Biscay, Spain). |
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Keywords: | PAH Extraction Ultrasonic probe Biota samples |
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