| 基于2,6-吡啶二甲酸聚合膜固定纳米金胶的过氧化氢传感器的研究 |
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| 引用本文： | 李春香,阳明辉,沈国励,俞汝勤.基于2,6-吡啶二甲酸聚合膜固定纳米金胶的过氧化氢传感器的研究[J].化学学报,2004,62(17):1663-1667. |
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| 作者姓名： | 李春香 阳明辉 沈国励 俞汝勤 |
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| 作者单位： | 湖南科技大学化学化工学院,湘潭,411201;湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,长沙,410082 |
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| 基金项目： | 国家自然科学基金 (Nos.2 0 0 750 0 6 ,2 99750 0 6)，湖南省自然科学基金 (Nos.0 1JJY1 0 0 3,0 1JJY2 0 99)，博士点基金 (No .2 0 0 1 0 532 0 0 8)资助项目 |
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| 摘 要： | 构造了一种以2,6-吡啶二甲酸电聚合膜为基底,以共价键合的半胱胺固定的纳米金胶吸附辣根过氧化物酶的过氧化氢生物传感器.在以对苯二酚作介体的条件下,实现电流的多级放大.该传感器与2,6-吡啶二甲酸聚合膜-辣根过氧化物酶修饰的传感器比较,具有更高的稳定性、更低的检测下限.在工作电位-0.1 V条件下,能快速催化还原过氧化氢.在传感器制作中,酶固定方法简单易行,制得的电极可多次重复使用,具有较好的使用价值.
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| 关 键 词： | 纳米金 2 6-吡啶二甲酸 半胱胺 聚合膜 |
Hydrogen Peroxide Sensor Based on Nano-Au Colloids Immobilized on 2,6-Pyridinedicarboxylic Acid Polymer |
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| LI Chun-Xiang,YANG Ming-Hui,SHEN Guo-Li,YU Ru-Qin.Hydrogen Peroxide Sensor Based on Nano-Au Colloids Immobilized on 2,6-Pyridinedicarboxylic Acid Polymer[J].Acta Chimica Sinica,2004,62(17):1663-1667. |
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| Authors: | LI Chun-Xiang YANG Ming-Hui SHEN Guo-Li YU Ru-Qin |
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| Institution: | 1. Chemistry and Chemical Engineering College, Hunan University of Science and Technology, Xiangtan 411201;2. State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University, Changsha 410082 |
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| Abstract: | A sensitive amperometric sensor for the determination of H 2O 2 has been constructed. Horseradish peroxidase was successfully immobilized on nano-Au colloids based on the thiol-tailed groups of cysteamine binded to the 2,6-pyridinedicarboxylic acid polymer. With the mediator of the hydroquinone in the solution, the sensor displayed excellent electrocatalytical response to the reduction of H 2O 2. Compared with enzyme-polymer modified sensor, the immobilization of enzyme was simple and the sensor exhibited a better stability and reproducibility. |
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| Keywords: | nano-Au colloid 2 6-pyridinedicarboxylic acid cysteamine polymer |
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