A dereplication strategy for the identification of new phenolic compounds from Anvillea radiata (Coss. & Durieu)

Phenolic compounds were selectively extracted from aerial parts of Anvillea radiata using Accelerated Solvent Extraction (ASE) in two steps. Given the two molecular families (flavonoids and germacranolides) described as present in the plant material, a first extraction step using chloroform as the extraction solvent was carried out to remove the germacranolides, the most abundant compounds. The minor phenolic compounds were then selectively extracted and enriched from the plant residue by methanol. Characterization of twenty five phenolic compounds in the methanolic extract was performed using HPLC-DAD-ESI-MS/MS and HPLC-HRMS analyses. Seven compounds corresponded to chlorogenic acid and dicaffeoylquinic acid derivatives and eighteen flavonoids (from which five aglycones and thirteen glycosides) were identified and some of them for the first time.The presence of these phenolic compounds, identified in the whole aerial parts, was then followed in each organ (flower, leave and stem). The chromatographic profiles of the stem and leave were very close, while the flower one was more different. However most of the compounds identified in aerial parts were recovered in each organ, mainly difference on peak intensity could be observed. The most abundant compound in flowers was found to be a di-caffeoylquinic acid derivative while isorhamnetin and spinacitin diglucoside derivatives were the most abundant ones in stems and leaves.