| 促血管生成素载体构建及在癌细胞中的表达 |
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| 引用本文： | 孙宇辉,史文静,赵怡璇,王梅,方凤奇,郭彩玲. 促血管生成素载体构建及在癌细胞中的表达[J]. 哈尔滨商业大学学报(自然科学版), 2004, 20(3): 266-269 |
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| 作者姓名： | 孙宇辉 史文静 赵怡璇 王梅 方凤奇 郭彩玲 |
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| 作者单位： | 吉林大学第二附属医院,妇产科,吉林,长春,130041;广州医学院附属第二医院妇产科,广州,510000;哈尔滨医科大学,微生物教研室,黑龙江,哈尔滨,l50086 |
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| 摘 要： | [目的]构建人促血管生成素-2(Ang-2)基因的重组peDNA3真核表达载体，为进一步研究Ang-2在肿瘤血管生长上的作用奠定基础．[方法]从人胎盘组织中提取Ang-2总RNA，经过RT-PCR扩增、TA克隆、酶切鉴定、DNA序列分析后，将纯化的Ang-2基因克隆到pcDNA3真核表达载体上，转染Hela细胞，应用RT-PCR方法鉴定细胞表达的mRNA．[结果]RT-PCR得到的产物经DNA测序，与Genebank中人Ang-2eDNA序列一致；构建人Ang-2真核表达载体，转染人Hela细胞，细胞表达Ang-2目的基因mRNA。[结论]成功构建人Ang-2基因真核表达载体pcDNA3-Ang-2．
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| 关 键 词： | 促血管生成素 基因 载体 构建 转染 |
| 文章编号： | 1672-0946(2004)03-0266-04 |
| 修稿时间： | 2004-04-02 |
Study on construction and expression of eukaryotic plasmid with human angiopoietin-2 cDNA in human cervical hela cells |
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| SUN Yu-hui,SHI Wen-jing,ZHAO Yi-xuan,WANG Mei,FANG Feng-qi,GUO Cai-ling. Study on construction and expression of eukaryotic plasmid with human angiopoietin-2 cDNA in human cervical hela cells[J]. Journal of Harbin University of Commerce :Natural Sciences Edition, 2004, 20(3): 266-269 |
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| Authors: | SUN Yu-hui SHI Wen-jing ZHAO Yi-xuan WANG Mei FANG Feng-qi GUO Cai-ling |
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| Affiliation: | SUN Yu-hui~1,SHI Wen-jing~2,ZHAO Yi-xuan~1,WANG Mei~1,FANG Feng-qi~1,GUO Cai-ling~3 |
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| Abstract: | Objective] Constructing recombinant eukaryotic expression vector coding for human Angiopoietin-2 in order to investigate the role of Ang-2 in carcinoma angiogenesis . [Methods] Extracting mRNA from human placenta;Identification the gene through RT-PCR,TA clone,restrict enzymes and DNA sequence analysis ;connecting Ang-2 to pcDNA3 and transfecting human hela cell;RT-PCR to identify mRNA expression. [Results] The gene sequence is identical with that reported in genebank.RT-PCR result indicated that recombinant plasmid expressed in hela cell.[Conclusion] Sucessfully constructing Ang-2 eukaryotic express vectors. |
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| Keywords: | Angiopoietins gene vector construction transfection |
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