邻近标记方法表征细胞-细胞相互作用

细胞-细胞相互作用在多种生理过程中有重要意义,针对这些相互作用的研究是化学与生命科学交叉领域的前沿热点.基于对酶的功能改造、定向进化和精准的时空调控,酶介导的邻近标记方法在分析细胞-细胞相互作用方面具有广泛的应用前景.本文重点描述了现有邻近标记方法的原理、效果、优缺点及潜在应用.

Proximity Labeling in Cell-cell Interaction Detection

Cell-cell interactions play an important role in many physiological processes. Elucidating these interactions is current interest in the frontier of interdisciplinary studies integrating of chemistry and life sciences. Enzymatic intercellular proximity labeling has become one of the most promising detection methods for cell-cell interactions， enabled by the rapid advancement of protein engineering techniques， including protein function manipulation， directed evolution of enzymes， and precise spatiotemporal regulation on enzymatic activities. We here describe some of the current proximity labeling designs， highlight key advantages and limitations of each method， and discuss the potential applications of proximity labeling.