表面修饰探针纳升电喷雾质谱脂质组学对大型溞和蚤状溞的快速鉴别

采用表面修饰探针敞开式纳升电喷雾质谱技术建立了脂质组学方法,以实现溞属(Daphnia)生物大型溞和蚤状溞的快速鉴别.采用微尺度表面修饰探针直接从Daphnia体内萃取和富集出脂质化合物,然后在常压敞开条件下进行纳升电喷雾质谱分析,利用高分辨率质谱仪获得脂质指纹图谱并进行脂质种类和结构鉴定.采用该方法获得了大型溞和蚤状溞的脂质指纹图谱,并研究了大型溞和蚤状溞体内的脂质组成特征.通过结合化学计量学手段实现了大型溞和蚤状溞的快速区分,并鉴定了相关的生物标志物.结果表明,所建立的微萃取探针纳升电喷雾质谱脂质组学方法具有分析速度快、灵敏度高的优点,可用于Daphnia属生物的快速鉴定和识别.

Rapid Classification of Daphniamagna and Daphnia pulex by Surface-coated Probe Nanoelectrospray Ionization Mass Spectrometry Lipidomics

A surface-coated probe nanoelectrospray mass spectrometry(SCP-nanoESI-MS) based lipidomics method was developed for the rapid species identification of Daphnia genus. A microscale surface-coated probe was used to extract and enrich the lipids from Daphnia, and the lipids enriched on the probe were then analyzed by nanoelectrospray ionization mass spectrometry under ambient and open-air conditions. Structural identification of the lipid species was achieved via high resolution MS analysis. By using the proposed method, the lipidomics fingerprints of D. magna and D. pulex were obtained, and the characteristics of lipid compositions from D. magna and D. pulex were also investigated. Rapid classification of D. magna and D. pulex as well as the identification of lipid markers were achieved via chemometrics. The results showed that the proposed SCP-nanoESI-MS lipidomics method has the advantages of fast analysis speed and high sensitivity, and can be used for rapid identification of Daphnia species.