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| TOTAL SYNTHESIS OF YEAST ALANINE TRANSFER RIBONUCLEIC ACID | |
| 作者姓名： | 王德宝 郑可沁 裘慕绥 梁镇和 吴仁龙 陈常庆 汪恩壁 朱莹书 申庆祥 余允华 汪猷 陈海宝 杨再完 陆蕴华 陈慎 王贵海 胡美浩 |
| 作者单位： | Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Cell Biology，Academia Sinica，Shanghai Institute of Cell Biology，Academia Sinica，Shanghai Institute of Cell Biology，Academia Sinica，Shanghai Institute of Cell Biology，Academia Sinica，Shanghai Institute of Organic Chemistry，Academia Sinica，Shanghai Institute of Organic Chemistry，Academia Sinica，Shanghai Institute of Organic Chemistry，Academia Sinica，Shanghai Institute of Organic Chemistry，Academia Sinica，Institute of Biophysics，Academia Sinica，Beijing，Institute of Biophysics，Academia Sinica，Beijing，Department of Biology，Peking University |
| 摘 要： | By a combination of chemical and enzymatic methods, small oligonucleotides with lengths varying from 2 to 8 nucleotides were synthesized from mononucleotides. The small oligonucleotides were then ligated with T_4 RNA ligase into six laxge ligonucleotides (9 to 19 nucleotides long) which were further ligated to form two half molecules with 35 and 41 nucleotides respectively, Finally, the two synthetic half molecules were annealed and ligated to obtain the whole molecule of yeast alanine tRNA (tRNA_y~(Ala)). Prior to this, two semi-syntheses were performed, i. e. ligation of the synthetic 5'-half molecule with the natural 3'-half molecule and that of the natural 5'-half molecule with the synthetic 3'-half molecule. |
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