Cytotoxicity and DNA Damage Effect of TGA-capped CdTe Quantum Dots |
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Authors: | LI Yan-bo ZHANG Hai-xia GUO Cai-xia HU Gui-qin DU Hai-ying JIN Ming-hua HUANG Pei-li SUN Zhi-wei YANG Wen-sheng |
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Institution: | 1. School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, P. R. China;
2. Public Health Institute, Beijing Chaoyang District Centre for Disease Control and Prevention, Beijing 100021, P. R. China;
3. School of Public Health, Jilin University, Changchun 130021, P. R. China;
4. Key Laboratory of Surface and Interface Chemistry of Jilin Province, College of Chemistry, Jilin University, Changchun 130012, P. R. China |
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Abstract: | The cytotoxicity and DNA damage caused by thioglycolic acid(TGA)-capped cadmium telluride(CdTe) quantum dots(QDs) to hepatocyte line HL-7702 were investigated. Cell viability was measured by 3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay; DNA damage was detected by single cell gel electro-phoresis(SCGE); the change of cell cycle progression was examined by propidium iodide(PI)-flow cytometry(FCM); apoptosis was measured by acridine orange/ethidium bromide(AO/EB) assay and Annexin V-FITC/PI-FCM(FITC: fluorescein isothiocyanate). The results show that the cytotoxicity induced by CdTe QDs was increased in a dose-dependent and time-dependent manner; after exposure to QDs for 24 h, as the exposure dose increased, the rate of DNA damage was significantly increased(P<0.05), and the degree of DNA damage was elevated. As the dose of CdTe QDs increased, the percentage of G0/G1 phase cells was significantly decreased(P<0.001), while the percent- tages of S and G2/M phases cells were significantly increased(P<0.001). In AO/EB assay, apoptotic cells could be observed under a fluorescence microscope, and apoptotic rate was increased as exposure dose increased. In Annexin V-FITC/PI-FCM assay, the apoptotic rates of CdTe QDs treated groups were significantly increased compared with that of control group(P<0.05). Our studies indicate that CdTe QDs could influence cell viability, and induce DNA damage, the S and G2/M phases arrest and apoptosis of HL-7702. |
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Keywords: | Apoptosis CdTe quantum dot(QD) Cell cycle Cell viability DNA damage |
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