Hydrolytically stable, diaminocarboxylic acid-based membranes buffering in the pH range from 6 to 8.5 for isoelectric trapping separations |
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Authors: | Fleisher Helen C Vigh Gyula |
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Affiliation: | Department of Chemistry, Texas A&M University, College Station, TX 77842-3012, USA. |
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Abstract: | Diaminocarboxylic acid carrier ampholytes, such as L-histidine, 2,3-diaminopropionic acid, L-ornithine, and L-lysine, were reacted with glycerol-1,3-diglycidyl ether (GDGE) and poly(vinyl alcohol) (PVA) in the presence of sodium hydroxide to produce hydrolytically and mechanically stable hydrogels, supported on a PVA substrate, for use as buffering membranes in isoelectric trapping (IET) separations. The pH values of the DACAPVA membranes were determined with the help of small-molecule pI markers and proteins and were found to be in the 6 < pH < 8.5 range. The membranes were successfully used to isoelectrically trap small ampholytes, desalt ampholyte solutions in IET mode, and effect the binary separation of chicken egg white proteins. |
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Keywords: | Buffering membranes Diaminocarboxylic acids Immobilized carrier ampholytes Isoelectric focusing Isoelectric membranes Isoelectric trapping Poly(vinyl alcohol) hydrogels |
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