Determination of one-dimensional distribution of14C-labelled compounds |
| |
| Authors: | R. Rexa I. Kron P. Kralik R. Tykva |
| |
| Affiliation: | (1) Department of General Biology, Faculty of Science, P.J. Safarik University, 041 67 Ko!["scaron"]("/content/f51648h4q24pk446/xlarge353.gif") ice, Czechoslovakia;(2) Institute of Experimental Medicine, P.J. Safarik University, Koice, Czechoslovakia;(3) Department of Physical and Analytical Chemistry, Faculty of Science, P.J. Safarik University, Koice, Czechoslovakia;(4) Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague, Czechoslovakia |
| |
| Abstract: | Three methods of determination of one-dimensional distribution of14C-labelled compounds are compared: measurement with position-sensitive detector /LB 282/511/, liquid scintillation counting /LSC/, and autoradiography with densitometric quantification. For a mutual comparison of these methods samples of blood serum were used, in which the fractional esterification rate /FER/ was determined by means of LCAT Test. The agreement of the methods of FER determination on the basis of the measurement with LB 282/511 or LSC was checked. In the case of autoradiography a correction for non-linearity between the blackening and the number of  -particles striking the area unit was necessary. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
