The advantages of evaporative light scattering detection in pharmaceutical analysis by high performance liquid chromatography and supercritical fluid chromatography

The evaporative light scattering detector (ELSD) has been used in pharmaceutical analysis by liquid and supercritical fluid chromatography. An ELSD equipped with interchangeable interfaces enables the use of various eluents (UV- or non-UV-absorbing) in isocratic or gradient mode. Analyses were performed on several non-UV-absorbing excipients and active substances. The narrow spread of the response factors of the various compounds investigated has indicated that the detector is suitable for direct raw quantitation of unknown samples in stability studies.