Elevated temperature-extended column length conventional liquid chromatography to increase peak capacity for the analysis of tryptic digests |
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Authors: | Sandra Pat Vanhoenacker Gerd |
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Institution: | Research Institute for Chromatography (R.I.C.), Kortrijk, Belgium. pat.sandra@richrom.com |
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Abstract: | High efficiency separations (200 000 plates) were obtained on conventional LC equipment by coupling 8 x 25 cm x 2.1 (or 4.6) mm id x 5 microm d(p) ODS columns (total length 2 m) and operation at 60 degrees C using a dedicated LC oven. The peak capacity in this 1-D set-up was 900 for the separation of human serum tryptic peptides analyzed after depletion of six highly abundant proteins. The chromatographic performance of an elevated temperature-extended column length conventional LC is highlighted. |
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