Magnetic assembled electrochemical platform using Fe2O3 filled carbon nanotubes and enzyme

A novel electrochemical nanostructured biosensor based on carbon nanotubes (CNTs) has been constructed by magnetic assembly method. The magnetic multi-walled carbon nanotubes (M-MWNTs) were prepared by introducing Fe₂O₃ nanoparticles into the nanotubes. Thus the multilayered functional platform could be assembled with the aid of magnetic field. The horseradish peroxidase (HRP) was employed as a model enzyme to demonstrate the final performance of the nanostructured biosensor. SEM, UV–vis spectroscopy and electrochemical techniques were used for characterization of assembly process. The resulting three-dimensional M-MWNTs/HRP multilayer films have showed satisfactory stability, biocompatibility and electrochemical properties.