High-performance liquid chromatographic determination of histamine N-methyltransferase activity |
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| Authors: | H Fukuda A Yamatodani I Imamura K Maeyama T Watanabe H Wada |
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| Institution: | Department of Pharmacology II, Osaka University, Faculty of Medicine, Japan. |
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| Abstract: | A method for the determination of histamine N-methyltransferase (HMT) activity by high-performance liquid chromatography based on post-column derivatization with omicron-phthalaldehyde is described. The determination involves the separation of the substrate, histamine, from its product. N tau-methylhistamine, using a weak cation exchanger, followed by on-line derivatization of these imidazoleamines with omicron-phthalaldehyde and their detection and quantitation with a fluorimetric detector. This assay method is suitable for the measurement of HMT activity during enzyme purification. |
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